Innotus obliquus extract (Chaga extract) induces G1 cell cycle arrest in HT-29 human colon cancer cells
Innotus obliquus extract include anticancer ingredients! Chaga mushroom ( Inonotus Obliquus ) is a white rot fungus which mainly grow in north of China, Russuia and etc. For the chaga mushroom commonly used as the chaga tea, innotus obliquus extract , ingection, syrup, aerosol and etc. The most important as a folk medicine for treating cancer in China, Russia, North America for many years.
Studies & researchings have reported that the innotus obliquus extract ( chaga extract ) contains rich nutrition ingredients like polysaccharides, polyphenols ( triterpenoids, steroids, ergosterol peroxides, inotodial and 3β-hydroxy-lanosta-8,24-dien-21-al, a lignin-like substance. With these rich nutrition ingreidients, the innotus obliquus extract show the effective biological activities as antioxidant, hepatoprotective, anti viral, anti inflammatory, anti tumor, platelet aggregation inhibitory. However, the molecular mechanisms responsible for the anti-cancer effects of I. obliquus are not well understood, despite its increasing usage.
In this study, we mainly investigated and examine the effects of ethanol extract of innotus obliquus extract on cell cycle progression of HT-29 cells, as well as its molecular mechanism in colon cancer cells, HT-29 human colon cancer cells were cultured in the presence of 2.5 – 10 µg/mL of EEIO, and analyzed the cell cycle arrest by flow cytometry and the cell cycle controlling protein expression by Western blotting.
Treatment cells with 2.5 – 10 µg/mL of EEIO reduced viable HT-29 cell numbers and DNA synthesis, increased the percentage of cells in G1 phase, decreased protein expression of CDK2, CDK4, and cyclin D1, increased expression of p21, p27, and p53, and inhibited phosphorylation of Rb and E2F1 expression. Among I. obliquus fractions, fraction 2 (fractionated by dichloromethane from EEIO) showed the same effect as EEIO treatment on cell proliferation and cell cycle-related protein levels. These results show and certified that fraction 2 is the major fraction that induces G1 arrest and inhibits cell proliferation, suggesting chaga mushroom ( Inonotus obliquus ) could be used as a natural anti-cancer ingredient in the food or pharmaceutical industry.
Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388940/
